Two-year field monitoring shows little evidence that transgenic potato containing ABF3 significantly alters its rhizosphere microbial community structure

Table 2 Results of permutational ANOVAs to investigate the effects of plant line (line), sampling time (time) and year (year) on the structure of rhizosphere microbial communities associated with untransformed and transgenic potatoes grown in an experimental field in 2009 and 2010. Analyses were based on Sørenson similarity matrices of square root arcsine-transformed data. All tests utilized 9999 permutations under the reduced model. Significant p values are italicized

Source	df	SS	MS	Pseudo-F	P(perm)	Unique perms	P(MC)
(a) Bacteria
Line	1	1623.2	1623.2	1.453	0.2987	799	0.2465
Year	1	23,139	23,139	3.9106	0.3356	3	0.0271
Time (year)	2	11,834	5916.9	3.9862	0.0001	9902	0.0001
Line × year	1	1908.3	1908.3	1.7082	0.2385	799	0.1775
Line × time (year)	2	2234.4	1117.2	0.75264	0.7867	9896	0.7033
Residuals	16	23,750	1484.4
Total	23	64,488
(b) Fungi
Line	1	1623.2	1623.2	1.453	0.2987	799	0.2465
Year	1	23,139	23,139	3.9106	0.3356	3	0.0271
Time (year)	2	11,834	5916.9	3.9862	0.0001	9902	0.0001
Line × year	1	1908.3	1908.3	1.7082	0.2385	799	0.1775
Line × time (year)	2	2234.4	1117.2	0.75264	0.7867	9896	0.7033
Residuals	16	23,750	1484.4
Total	23	64,488

df degrees of freedom, P(MC) Monte Carlo p value used when the number of unique perms was too low

ISSN: 2288-1220