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Table 2 Results of permutational ANOVAs to investigate the effects of plant line (line), sampling time (time) and year (year) on the structure of rhizosphere microbial communities associated with untransformed and transgenic potatoes grown in an experimental field in 2009 and 2010. Analyses were based on Sørenson similarity matrices of square root arcsine-transformed data. All tests utilized 9999 permutations under the reduced model. Significant p values are italicized

From: Two-year field monitoring shows little evidence that transgenic potato containing ABF3 significantly alters its rhizosphere microbial community structure

Source df SS MS Pseudo-F P(perm) Unique perms P(MC)
(a) Bacteria
 Line 1 1623.2 1623.2 1.453 0.2987 799 0.2465
 Year 1 23,139 23,139 3.9106 0.3356 3 0.0271
 Time (year) 2 11,834 5916.9 3.9862 0.0001 9902 0.0001
 Line × year 1 1908.3 1908.3 1.7082 0.2385 799 0.1775
 Line × time (year) 2 2234.4 1117.2 0.75264 0.7867 9896 0.7033
 Residuals 16 23,750 1484.4     
 Total 23 64,488      
(b) Fungi
 Line 1 1623.2 1623.2 1.453 0.2987 799 0.2465
 Year 1 23,139 23,139 3.9106 0.3356 3 0.0271
 Time (year) 2 11,834 5916.9 3.9862 0.0001 9902 0.0001
 Line × year 1 1908.3 1908.3 1.7082 0.2385 799 0.1775
 Line × time (year) 2 2234.4 1117.2 0.75264 0.7867 9896 0.7033
 Residuals 16 23,750 1484.4     
 Total 23 64,488      
  1. df degrees of freedom, P(MC) Monte Carlo p value used when the number of unique perms was too low